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MARTINO Gianvito |
Tel.: +39 (02) 2643 4853 (4854/4867)
Fax: +39 (02) 2643 4855
e-mail: g.martino@hsr.it |
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Address |
Neuroimmunology Unit
Dept. of Neuroscience
San Raffaele Scientific Institute
DIBIT, Via Olgettina 58
20132 Milan, ITALY
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Research themes |
Role of inflammatory
mediators (cytokines, chemokines, cystein proteases) and T cells in
immune-mediated diseases of the central nervous system (CNS) and neuromuscular
diseases; development of gene-therapy approaches for demyelinating diseases
(cytokine-based and neuroprotective treatments) |
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Key words |
Inflammatory mediators
(cytokines, chemokines, cystein proteases), T cells, viral vectors,
gene-therapy, demyelination, remyelination, trophic factors, tumors, EAE,
EAMG |
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Methods |
Cell cultures (bulk,
primary, organotypic, lines), cellular immunology (T cell proliferation and
cytotoxic assays, FACS analysis), molecular biology (Southern and Northern
blotting, recombinant DNA techniques), cytokine detection systems (ELISA,
ICS, TaqmanÆ, ISH), chemokine and chemokine receptor detection systems
(TaqmanÆ), immunocytochemistry, animal handling and manipulation
(stereotaxis, EAE, EAMG, microsurgery) |
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Composition of the research group |
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Alessandra BERGAMI |
Lab Technician |
San Raffaele Scientific Institute |
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Elena BRAMBILLA |
BS, Biologist |
San Raffaele Scientific Institute |
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Roberto FURLAN |
MD, PhD |
San Raffaele Scientific Institute |
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Maira GIRONI |
MD, Neurologist |
San Raffaele Scientific Institute |
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Gianvito MARTINO |
MD, Neurologist |
San Raffaele Scientific Institute |
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Stefano PLUCHINO |
MD, PhD Student |
San Raffaele Scientific Institute |
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Francesca RUFFINI |
BS, Biologist |
San Raffaele Scientific Institute |
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Recent publications
- Cortese I, Tafi R, Grimaldi LME, Martino G, Nicosia A, Cortese R (1996) Identification of peptides specific for cerebrospinal fluid antibodies in multiple sclerosis by using phage libraries. Proc. Natl. Acad. Sci. USA, 93:11063-11067.
- Martino G, Grohovaz F, Brambilla E, Codazzi F, Consiglio A, Clementi E, Filippi M, Comi G, Grimaldi LME (1998) Proinflammatory cytokines regulate antigen-independent T-cell activation by two separate calcium-signaling pathways in multiple sclerosis patients. Ann. Neurol., 43:340-349.
- Filippi M, Rocca MA, Martino G, Horsfield MA, Comi G (1998) Magnetization transfer changes in the normal-appearing white matter precede the appearance of enhancing lesions in patients with multiple sclerosis. Ann. Neurol., 43:809-814.
- Moiola L, Galbiati F, Martino G, Amadio S, Brambilla E, Comi G, Vincent A, Grimaldi LME, Adorini L (1998) IL-12 is required for the induction of experimental autoimmune myasthenia gravis, an antibody-mediated disease. Eur. J. Immunol., 28:2487-2497.
- Furlan R, Poliani PL, Galbiati F, Bergami A, Grimaldi LME, Comi G, Adorini L, Martino G (1998) Central nervous system delivery of interleukin-4 by a non-replicative herpes simplex type 1 viral vector ameliorates autoimmune demyelination. Hum. Gene Ther., 9:2605-2617.
- Martino G, Hartung HP (1999) Immunopathogenesis of multiple sclerosis: the role of T cells. Curr. Opin. Neurol., 12:309-321.
- Furlan R, Martino G, Galbiati F, Poliani PL, Smiroldo S, Bergami A, Desina G, Comi G, Flavell R, Su M, Adorini L (1999) Caspase-1 regulates the inflammatory process leading to autoimmune demyelination. J. Immunol., 163:2403-2409.
- Del Maschio A, De Luigi A, Martin-Padura I, Brockhaus M, Bartfai T, Fruscella P, Adorini L, Martino G, Furlan R, De Simoni MG, Dejana E (1999) Leukocyte recruitment in the cerebrospinal fluid of mice with experimental meningitis is inhibited by an antibody to junctional adhesion molecule (JAM). J. Exp. Med., 190:1351-1356.
- Mattner F, Smiroldo S, Galbiati F, Muller M, Di Lucia P, Poliani PL, Martino G, Panina-Bordignon P, Adorini L (2000) Inhibition of Th1 development and treatment of chronic-relapsing experimental allergic encephalomyelitis by a non-hypercalcemic analogue of 1,25-dihydroxyvitamin. Eur. J. Immunol., 30:498-508.
- Martino G, Poliani Pl, Marconi P, Comi G, Furlan R (2000) Cytokine gene therapy of autoimmune demyelination revisited using herpes simplex virus type-1-derived vectors. Gene Ther., 7:1087-1093.
- Martino G, Furlan R, Brambilla E., Bergami A., Ruffini F, Gironi M, Poliani PL, Grimaldi LME, Comi G (2000) Cytokines and Immunity in Multiple Sclerosis: the Dual Signal Hypothesis. J. Neuroimmunol., 109:3-9.
- Furlan R, Bergami A, Lang R, Brambilla E, Franciotta D, Martinelli V, Comi G, Panina P, Martino G (2000) Interferon-b treatment in multiple sclerosis patients decreases the number of circulating cells producing IFN-g and IL-4. J. Neuroimmunol., 111:86-92.
- FURLAN R, POLIANI PL, MARCONI PC, BERGAMI A, RUFFINI F, ADORINI L,
GLORIOSO JC, COMI G, MARTINO G (2001) Central nervous system gene therapy
with interleukin-4 inhibits progression of ongoing relapsing-remitting
autoimmune encephalomyelitis in Biozzi AB/H mice. Gene Ther., 8:13-19.
- FRANCIOTTA DM, MARTINO G, ZARDINI E, FURLAN R, BERGAMASCHI R, ANDREONI
L, COSI V (2001) Serum and CSF levels of MCP-1 and IP-10 in multiple
sclerosis patients with acute and stable disease and undergoing
immunomodulatory therapies. J. Neuroimmunol., 115:192-198.
- POLIANI PL, BROK H, FURLAN R, RUFFINI F, BERGAMI A, DESINA G, MARCONI
PC, ROVARIS M, UCCELLI A, GLORIOSO JC, PENNA G, ADORINI L, COMI G, t'HART
B, MARTINO G (2001) Delivery of a non-replicative herpes simplex type-1
vector engineered with the IL-4 gene to the central nervous system protects
rhesus monkeys from hyperacute autoimmune encephalomyelitis. Hum Gene
Ther., 12:905-920.
- FURLAN R, BRAMBILLA E, RUFFINI F, POLIANI PL, BERGAMI A, MARCONI PC,
FRANCIOTTA DM, PENNA G, COMI G, ADORINI L, MARTINO G (2001) Intrathecal
delivery of IFNg protects C57BL/6 mice from chronic-progressive
experimental autoimmune encephalomyelitis by increasing apoptosis of
CNS-infiltrating lymphocytes. J Immunol., 167:1821-1829.
- MARTINO G, FURLAN R, COMI G, ADORINI L (2001) The ependymal route to
the CNS: an emerging gene therapy approch for MS. Trends Immunol.,
22:483-490.
- RUFFINI F, FURLAN R, POLIANI PL, BRAMBILLA E, MARCONI PC, BERGAMI A,
DESINA G, GLORIOSO JC, COMI G, MARTINO G (2001) Fibroblast growth factor-II
gene therapy reverts the clinical course and the pathological signs of
chronic experimental autoimmune encephalomyelitis in C57BL/6 mice. Gene
Ther., 8:1207-1213.
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