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PROBERT Lesley
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Tel.: +30-1-6478866, 6478863
Fax: +30-1-6456547
e-mail:
lesley_probert@hol.gr
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Address
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Laboratory of Molecular Genetics
Hellenic Pasteur Institute
127 Vassilissis Sophias Avenue
115 21 Athens - HELLAS
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Research themes
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TNF family signaling pathways in immune function and neuro-immune interactions. Neuroprotection and brain repair mechanisms.
T cell differentiation and function. Gene expression profiling of CNS diseases.
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Key words
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TNF, TNF receptors, NF-kappaB, apoptosis, autoimmunity, multiple sclerosis, EAE,
stroke, neuroprotection, transgenic, knockout, conditional gene targeting, CNS, T cells, neurons
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Methods
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Most methodologies in molecular and cellular biology (DNA analysis and manipulation, RNA and protein expression analysis, cellular immunology, immunocytochemistry,
cell culture), expression of transgenes in cell lines and in mice, deletion or mutation of genes in mice, conditional gene targeting technology in vivo,
microarrays, mouse models of autoimmunity, inflammation and neurodegeneration.
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Composition of the research group
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Sylva Haralambous
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B.Sc, Ph.D, Biologist
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Era Taoufik
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B.Sc, M.Sc, Biologist
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Vivi Tseveleki
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B.Sc, M.Sc, Biochemist
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Voula Lambropoulou
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B.Sc, Medical Technologist
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Maria Eugenidou
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B.Sc, Biologist
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Angelos Vagionas
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M.Vet.Sci., Animal Technician
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Recent publications
- TSEVELEKI V, BAUER J, TAOUFIK E, RUAN C, HARALAMBOUS S, LASSMANN H & PROBERT L. c-FLIPL overexpression in T cells
is sufficient to drive Th2 effector responses and immunoregulation of experimental autoimmune encephalomyelitis.
Submitted for publication January 2004.
- AKASSOGLOU K, BAUER J, TSEVELEKI V, LASSMANN H, PROBERT L & STRICKLAND S. Fibrin depletion decreases inflammation
and delays the onset of demyelination in a in a tumor necrosis factor transgenic model offor multiple sclerosis.
Proc. Natl. Acad. Sci. USA, in press February 2004.
- AKASSOGLOU K, DOUNI E, BAUER J, LASSMANN H, KOLLIAS G & PROBERT L (2003).Exclusive tumor necrosis factor signaling through
the p75TNF receptor triggers inflammatory ischaemia in the central nervous system of transgenic mice. Proc. Natl. Acad. Sci. USA, 100, 709-714.
- PROBERT L, AKASSOGLOU K (2001). Glial expression of cytokines in transgenic animals - how do these models reflect the "normal situation".
Invited Review. Glia, 36, 212-219.
- PROBERT L, EUGSTER H-P, AKASSOGLOU K, BAUER J, FREI K, LASSMANN H & FONTANA A (2000). TNFR1 signalling is critical for
demyelination and the limitation of T-cell responses during immune-mediated CNS disease. Review, Brain, 123, 2005-2019.
- KASSIOTIS G, BAUER J, AKASSOGLOU K, LASSMANN H, KOLLIAS G & PROBERT L (1999). A tumor necrosis factor-induced model
of human primary demyelinating diseases develops in immunodeficient mice. Eur. J. Immunology 29: 912-917.
- KASSIOTIS G, PASPARAKIS M, KOLLIAS G & PROBERT L (1999).TNF accelerates the onset but does not alter the incidence and severity
of myelin basic protein-induced experimental autoimmune encephalomyelitis. Eur. J. Immunology 29: 774-780.
- AKASSOGLOU K, BAUER J, KASSIOTIS G, PASPARAKIS M, LASSMANN H, KOLLIAS G & PROBERT L (1998). Oligodendrocyte apoptosis and
primary demyelination induced by local TNF/p55TNF receptor signaling in the CNS of transgenic mice: Models for Multiple
Sclerosis with primary oligodendropathy. Am. J. Pathol. 153: 801-813.
- AKASSOGLOU K, PROBERT L, KONTOGEORGOS G & KOLLIAS G (1997) Astrocyte- but not Neuron-Specific Transmembrane TNF
Triggers Inflammation, Demyelination and Neuronal Degeneration in the CNS of Transgenic Mice. J. Immunol. 158: 438-445.
- PROBERT L, AKASSOGLOU K, PASPARAKIS M, KONTOGIORGOS G & KOLLIAS G (1995). Spontaneous inflammatory demyelinating disease
in transgenic mice showing CNS-specific expression of tumor necrosis factor. Proc. Natl. Acad. Sci. USA 92: 11294-11298.
- PROBERT L, PLOWS D, KONTOGEORGOS G & KOLLIAS G. (1995) The type I IL-1 receptor acts in series with TNF to induce
arthritis in TNF transgenic mice. Eur. J. Immunol. 25: 1794-1797.
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