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RIECKMANN Peter

Tel.: +49-931-201-23766

Fax: +49-931-201-23488

e-mail: p.rieckmann@mail.uni-wuerzburg.de

 

Address

Clinical Research Group for Multiple Sclerosis and Neuroimmunology

Department of Neurology, Julius-Maximilians-Universität

Josef-Schneider-Str. 11

D-97080 Würzburg, Germany

Research themes

Role of soluble biological mediators (cytokines, chemokines, neurotrophins) in inflammatory diseases of the central nervous system (CNS)

Functional genomics of multiple sclerosis

Molecular and functional aspects of the blood brain barrier in diseases of the CNS

Key words

Inflammatory mediators (cytokines, chemokines), functional polymorphisms, blood brain barrier, cerebral endothelial cells, neurotrophic factors, stem cells, trophic support, multiple sclerosis, molecular imaging

Methods

Cell cultures (bulk, primary, organotypic, lines), primary human cerebral endothelial cell and microglia cultures, murine neural stem cell cultures, cellular immunology (T cell proliferation and cytotoxic assays, FACS analysis), cell adhesion and transmigration assays, protein analyses (PAGE, IEF, Western blotting), molecular biology (Southern and Northern blotting, EMSA, RNAse protection assays, recombinant DNA techniques, gene arrays, quantitative RT PCR, allelic discrimination PCR), cytokine detection systems (ELISA, ELIspot, quantitative RT-PCR, ISH), chemokine and neurotrophic factor detection systems (RT-PCR, ISH), immunocytochemistry, confocal microscopy

Projects

Expression profiling of human cerebral endothelial cells

Immunoregulation at the blood brain barrier

Functional polymorphisms of candidate genes in MS

Composition of the research group

Daniela KRÄMER

Lab Technician

Dep. of Neurology, MS Research Group

Verena WENGEL

Lab Technician

Dep. of Neurology, MS Research Group

Bettina MEYER

Lab Technician

Dep. of Neurology, MS Research Group

Petra HAUCK

Lab Technician

Dep. of Neurology, MS Research Group

Vera HUMMEL

PhD, Cell Biology

Dep. of Neurology, MS Research Group

Na-Young LEE

PhD, Pharmacology

Dep. of Neurology, MS Research Group

Antje KRONER

MD, Neurologist, Molecular Biology

Dep. of Neurology, MS Research Group

Boris KALLMANN

MD, Neurologist, Cell Biology

Dep. of Neurology, MS Research Group

Mathias BUTTMANN

MD, Neurologist, Molecular Biology

Dep. of Neurology, MS Research Group

Klemens RUPRECHT

MD, Neurologist, Virology

Dep. of Neurology, MS Research Group

Felix GRONEN

MS, Neurologist

Dep. of Neurology, MS Research Group

Mathias MÄURER

MD, Neurologist, Molecular imaging

Dep. of Neurology, MS Research Group

Peter RIECKMANN

MD, Neurologist

Dep. of Neurology, MS Research Group

Recent publications

  1. KRUSE N, MORIABADI N, RIECKMANN P. Determination of early immunological responses in cultures of primary stimulated human peripheral mononuclear cells. J. Immunol. Methods 247:131-139, 2001.

  2. HUMMEL V, KALLMANN B, FÜLLER T, WAGNER BAYAS A.S., TONN J.C, BENVENISTE E, TOYKA K.V., RIECKMANN, P. Production of MMPs in human cerebral endothelial cells and their role in shedding of adhesion molecules. J. Neuropathol. Exp. Neurol. 60: 320-327, 2001.

  3. RUPRECHT K, KUHLMANN T, HUMMEL V, SEIF F, BRÜCK W, RIECKMANN P. Oncostatin M has immunoregulatory functions on human cerebral endothelial cells and is expressed in multiple sclerosis lesions. J. Neuropathol. Exp. Neurol. 60: 1087-1098, 2001.

  4. HACKSTEIN H., BITSCH A., BOHNERT A, HOFMANN H, WEBER F, LININGTON C, MÄURER M, POSER S, RIECKMANN P, BEIN G. The interleukin-4 receptor variant R551 influences to major clinical forms of multiple sclerosis. J. Neuroimmunol. 113: 240-248, 2001.

  5. MORIABADI N, NIEWIESK S, KRUSE N, WEISSBRIC B, JUNG S, TER MEULEN V, TOYKA K, RIECKMANN P. Influenca vaccination in MS: Absence of T-cell response against white matter proteins. Neurology 56: 938-943,2001.

  6. SHAPIRA L, STABHOLZ A, RIECKMANN P, KRUSE N. Genetic polymorphism in the TNF-promotor region in families with localized early-onset periodontitis J Periodontal Res. 36: 183-186, 2001.

  7. KRUSE N, BERG D, FRANCIS M.J., NAUMANN M, RAUSCH W.D., REINERS K, RIECKMANN P, WEISHAUPT A, BECKER G. Reduction of Menkes mRNA and copper in leukocytes of patients with primary adult-onset dystonia. Ann. Neurol. 49: 405-408, 2001.

  8. RIECKMANN P, SMITH K. Multiple Sclerosis: More than inflammation and demyelination. Trends Neurosci. 24; 435-437, 2001

  9. MÄURER M, LOSERTH S, KOLB-MÄURER A, PONATH A, WIESE S, KRUSE N, RIECKMANN P. A polymorphism in the human cytotoxic T-lymphocyte antigen 4 (CTLA-4) gene (exon 1 +49) alters T-cell activation Immunogenetics 54: 1-8, 2002.

  10. KAHL K.G., KRUSE N, TOYKA KV, RIECKMANN P. Serial analysis of cytokine mRNA profiles in whole blood samples from patients with early MS. J. Neurol. Sci. 200; 53-55, 2002.

  11. KAHL K.G., KRUSE N, FALLER H, WEIß H, RIECKMANN P. Expression of tumor necrosis factor-alpha and interferon-gamma mRNA in blood correlates with depression scores in patients with multiple sclerosis. Psychoneuroendocrinology 27; 671-81, 2002.

  12. FLACHENECKER P, JUNG S, RIECKMANN P, TOYKA K.V. sICAM-1 is not a marker for disease activity in the relapse-free interval of multiple sclerosis - a cross-sectional pilot study. J. Neurol. 249; 1001-1003, 2002.

  13. GIEß R, MÄURER M, GOLD R, TOYKA K.V., SENDTNER M, RIECKMANN P. A null mutation in the CNTF gene is associated with early onset of MS. Arch. Neurol 59, 407-409, 2002.

  14. LINKER R, MÄURER M, GAUPP S, MARTINI R, HOLTMANN B, GIESS R, RIECKMANN P, LASSMANN H, TOYKA K.V., SENDTNER M, GOLD R. CNTF is a major protective factor in demyelinating CNS disease: a neurotrophic cytokine as modulator in neuroinflammation. Nature Med. 8, 620-624, 2002.

  15. KALLMANN B, WAGNER S, HUMMEL V, BUTTMANN M, BAYAS A, TONN J.C., RIECKMANN P. Characteristic gene expression profile of human cerebral endothelial cells. FASEB Journal 16, 589-591, 2002.

  16. FLACHENECKER P, KÜMPFEL T, KALLMANN B, GOTTSCHALK M, GRAUER O, RIECKMANN P, TRENKWALDER C, TOYKA KV. Fatigue in multiple sclerosis: a comparison of different rating scales and correlation to clinical parameters. Multiple Sclerosis 8, 523-526, 2002.

  17. RUPRECHT K, STADELMANN C, HUMMEL V, KLEIN O, BRÜCK W, RIECKMANN P. Brain dervied neurotrophic factor does not act on adult human cerebral endothelial cells. Neurosci. Lett. 330, 175-178,2002.

  18. MÄURER M, PONATH A, KRUSE N, RIECKMANN P. CTLA4 exon 1 dimorphism is associated with primary progressive multiple sclerosis. J. Neuroimmunol. 131, 213-215, 2002.

  19. BAYAS A, HUMMEL V, KALLMANN B, KARCH C, TOYKA K.V, RIECKMANN P. Human cerebral endothelial cells are a potential source for bioactive BDNF. Cytokine 19, 55-58, 2002.

  20. MICHEL U, KALLMANN B, RIECKMANN P, ISBRANDT D. UM 9(5)h and UM 9(5)p, a human and a porcine non-coding transcript with preferential expression in the cerebellum. RNA 8, 1538-47, 2002.

  21. RIECKMANN P, MÄURER M. Anti-inflammatory strategies to prevent axonal damage in multiple sclerosis. Curr. Opin. Neurol.15: 361-370, 2002.

  22. MARTINO G, ADORINI L, RIECKMANN P, HILLERT J, KALLMANN B, COMI G, FILIPPI M. Inflammation in multiple sclerosis: the good, the bad and the complex. Lancet Neurology 1; 499-509, 2002

  23. WAGNER S, FUELLER T, HUMMEL V, RIECKMANN P, TONN J.C. Influence of VEGF-R2 inhibition on MMP secretion and motility of human microvascular cerebral endothelial cells (HCEC). J. Neuro-Oncology 62, 221-231, 2003.

  24. KOLB-MÄURER A, KÄMMERER U, MÄURER M, GENTSCHEV I, BRÖCKER E-B, RIECKMANN P, KÄMPGEN E. Production of IL-12 and IL-18 in human dendritic cells upon infection by Listeria monocytogenes. FEMS Immunol. Med. Microbiol. 35, 255-62, 2003.

  25. BAYAS A, KRUSE N, MORIABADI N, WEBER F, HUMMEL V, WOHLLEBEN G, GOLD R, TOYKA K.V., RIECKMANN P. Modulation of cytokine mRNA expression by brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF) in human immune cells. Neurosci. Lett. 335, 155-158, 2003.

  26. RIECKMANN P, ENGELHARDT B. Building up the blood brain barrier. Nature Med. 9: 10-11, 2003.

  27. KALLMANN B, SAUER S, SCHLIEßER M, WARMUTH-METZ M MD, FLACHENECKER P, BECKER G, RIECKMANN P, MÄURER M. Determination of ventricular diameters in multiple sclerosis patients with transcranial sonography (TCS) - a two year follow-up study. J. Neurol. (in press).

  28. BUTTMANN M, GOEBELER M, RIECKMANN P. Local pain during Rebif injection is not due to acidic pH. J. Neurol. Neurosurg. Psych. (in press).

  29. KRONER A, MÄURER M, LOSERTH S, KLEINSCHNITZ C, HEMMER B, ROSCHE B, TOYKA KV, RIECKMANN P. Analysis of the Monocyte- Chemoattractant- Protein 1 (MCP-1) -2518 promotor-polymorphism in patients with Multiple Sclerosis. Tissue Antigens (in press).

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