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STINISSEN Piet
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Tel.: +32 11 26 92 04
Fax: +32 11 26 92 09
e-mail:
piet.stinissen@luc.ac.be
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Address
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Biomedical Research Insitute
Limburg University Center
University Campus, Building A
B-3590 Diepenbeek - BELGIUM
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Research themes
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Role of autoreactive and regulatory T-cells in multiple sclerosis (functional reactivity, phenotype, T-cell receptor expression), development of experimental T-cell targeted immunotherapies
(T-cell vaccination), study of the interactions between autoreactive T-cells and oligodendrocytes and identification of novel MS markers. See:
http://www.luc.ac.be/biomed/english/
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Key words
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Autoreactive T-cells, myelin antigens, anticlonotypic T-cells, T-cell vaccination, regulatory T-cells, oligodendrocytes, serological antigen selection (SAS)
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Methods
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Cellular immunology (T cell isolation and cloning, proliferation and cytotoxic assays, FACS analysis), protein analyses (2D-PAGE, IEF, Western blotting), molecular biology
(DNA cloning, sequencing, PCR, immunoscope), cytokine quantification (ELISA, quantitative PCR), immunohistochemistry, immunogenetics (SNP's and microsatellites), apoptosis detection,
cDNA phage display, serological antigen selection (SAS)
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Composition of the research group
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Jef RAUS
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MD, PhD, Institute director
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Biomedical Research Institute - LUC
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Piet STINISSEN
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PhD, Head of Unit, Vice-director
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Biomedical Research Institute - LUC
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Niels HELLINGS
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PhD
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Biomedical Research Institute - LUC
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Veerle SOMERS
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MD, Neurologist
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Biomedical Research Institute - LUC
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Joris VANDERLOCHT
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PhD student
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Biomedical Research Institute - LUC
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Koen VENKEN
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PhD student
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Biomedical Research Institute - LUC
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Mariëlle THEWISSEN
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PhD student
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Biomedical Research Institute - LUC
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Loes LINSEN
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PhD student
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Biomedical Research Institute - LUC
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Kurt BAETEN
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PhD student
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Biomedical Research Institute - LUC
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Cindy GOVARTS
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PhD student
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Biomedical Research Institute - LUC
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Igna RUTTEN
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Lab technician
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Biomedical Research Institute - LUC
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Christel BOCKEN
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Lab technician
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Biomedical Research Institute - LUC
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Josianne BLEUS
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Lab technician
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Biomedical Research Institute - LUC
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Anne BOGAERS
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Study nurse / data manager
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Biomedical Research Institute - LUC
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Recent publications
- STINISSEN P., VANDEVYVER C., MEDEAR R., VANDEGAER L., NIES J., TUYLS L., HAFLER D., RAUS J. AND ZHANG J. (1995) Increased frequency of gd T cells in cerebrospinal fluid and peripheral blood of patients with multiple sclerosis:
Reactivity, cytotoxicity and T cell receptor rearrangements. J. Immunol. 154, 4886-4894
- MEDAER R, STINISSEN P, TRUYEN L, RAUS J, ZHANG J. (1995) Depletion of myelin basic protein autoreactive T-cells by T-cell vaccination: A pilot clinical trial in multiple sclerosis. Lancet 346, 807-808
- ZHANG J., VANDEVYVER C., STINISSEN P., AND RAUS J. (1995) In vivo clonotypic regulation of human myelin basic protein-reactive T cells by T cell vaccination. J. Immunol. 155, 5868-5877.
- HERMANS G., STINISSEN P., HAUBEN L., VAN DEN BERG-LOONEN E., RAUS J., ZHANG J. (1997) Cytokine Profile of Myelin Basic Protein-Reactive T Cells in Multiple Sclerosis and Healthy Individuals. Ann. Neurol., 42:18-27
- HERMANS G., U. DENZER, A. LOHSE, J. RAUS, AND P. STINISSEN.
(1999). Cellular and humoral immune responses against
autoreactive T cells in multiple sclerosis patients after
T cell vaccination. J. Autoimmun. 13, 233-246.
- JORENS P.G., A. VANDERBORGHT, B. CEULEMANS, H.P. VAN BEVER, L. L. BOSSAERT, M. IEVEN, H. GOOSSENS, P.M. PARIZEL, H. VAN DIJK, J. RAUS, P. STINISSEN. (2000). Encephalomyelitis-associated antimyelin
autoreactivity induced by streptococcal exotoxins. Neurol. 54, 1433-1441.
- HELLINGS N., M. BAREE, C. VERHOEVEN, M.B. D'HOOGHE, R. MEDAER, C.C.A. BERNARD, J. RAUS, P. STINISSEN. (2001). T-cell reactivity to multiple myelin antigens in multiple sclerosis patients and
healthy controls. J. Neurosc. Res. 63, 290-302.
- HELLINGS N., G. GELIN, R. MEDAER, L. BRUCKERS, Y. PALMERS, J. RAUS P. STINISSEN. (2002). Longitudinal study of antimyelin T-cell reactivity in relapsing-remitting multiple sclerosis: association with clinical and MRI activity. J. Neuroimmunol. 126, 143-160.
- BUNTINX M., P. STINISSEN, P. STEELS, M. AMELOOT, AND J. RAUS. (2002). Immune-mediated oligodendrocyte injury in multiple sclerosis: Molecular mechanisms and therapeutic interventions. Crit. Rev. Immunol. 22, 391-424.
- VAN DER AA A., N. HELLINGS, R. MEDAER, G. GELIN, Y. PALMERS, J. RAUS, AND P. STINISSEN. (2003). T cell vaccination in multiple sclerosis patients with autologous CSF-derived
activated T cells: results from a pilot study. Clin. Exp. Immunol. 131, 155-168.
- VAN DER AA A., N. HELLINGS, C.C. A. BERNARD, J. RAUS, AND P. STINISSEN. (2003). Functional properties of myelin oligodendrocyte glycoprotein-reactive T cells in multiple sclerosis patients
and controls. Journal of Neuroimmunology 137, 164-176.
- BUNTINX, M., M. MOREELS, F. VANDENABEELE, I. LAMBRICHTS, J. RAUS, P. STEELS, P. STINISSEN, AND M. AMELOOT. (2004). Cytokine-induced cell death in oligodendroglial cell lines. I. Synergistic effects
of IFN-g & TNF-a ?on apoptosis. In press
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